Manipulation of plant polysaccharide synthases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/00 (2006.01) C12N 15/29 (2006.01) C12N 15/52 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2461825

The present invention provides compositions and methods for manipulation of plant polysaccharides and plant polysaccharide synthases. Compositions include novel nucleotide sequences encoding polysaccharide synthases polypeptides. and biologically active variants thereof. Further provided are methods for polysaccharide manipulation using the sequences disclosed herein. One method comprises stably incorporating into the genome of a plant cell. a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence. Methods for enhancing digestibility of plants. improving gum extractability from plants. and altering plant growth are also provided.

Cette invention concerne des compositions et des procédés de manipulation de polysaccharides végétaux et de synthases de polysaccharides végétaux. Ces compositions contiennent des nouvelles séquences nucléotidiques codant pour des polypeptides de synthases de polysaccharides, et des variants biologiquement actifs de ceux-ci. La présente invention concerne également des procédés permettant de manipuler des polysaccharides à l'aide des séquences décrites dans l'invention. Un mode de réalisation consiste à incorporer de manière stable, dans le génome d'une cellule végétale, une séquence nucléotidique décrite dans l'invention, laquelle séquence est fonctionnellement liée à un promoteur hétérologue, puis à régénérer une plante transformée de manière stable et qui exprime ladite séquence nucléotidique. En outre, cette invention concerne des procédés permettant d'augmenter la digestibilité des plantes, d'améliorer l'extractibilité de la gomme sur les plantes et de modifier la croissance des plantes.

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