Monomer identification in sample mixtures of polymers

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2333916

Systems and methods for detecting monomer changes in a sample when an unknown quantity of expected monomers may also be present. Homogeneous and heterogeneous samples are exposed to polymer probes for hybridization. The hybridization affinities of the polymer probes to the samples are then compared to determine differences between the polymers in the samples. Accordingly, deletion, substitution and insertion mutations may be detected in a heterogeneous sample of nucleic acids.

L'invention porte sur des systèmes et procédés d'identification des modification de polymères dans des échantillons où peuvent également se trouver des quantités inattendues de monomères. A cette fin on expose des échantillons homogènes et hétérogènes à des sondes de polymères pour provoquer une hybridation. Les affinités d'hybridation desdites sondes avec les échantillons sont alors comparées pour déterminer les différences entre les polymères des échantillons, ce qui permet de détecter les mutations par suppression, substitution, et insertion dans un échantillon hétérogène d'acides nucléiques.

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