Peptide-presenting surfaces for long-term culture of...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) C12N 5/074 (2010.01) C12N 5/077 (2010.01)

Patent

CA 2739364

The present invention relates to methods of growing and maintaining pluripotent cells on an insoluble substrate that presents a peptide that binds to glycosaminoglycans, such as heparin. Specifically, methods of growing and maintaining pluripotent cells on substrates having a chemically defined surface presenting at least one peptide having basic amino acid residues separated by one or two hydrophobic amino acid residues.

La présente invention concerne des procédés pour faire croître et maintenir des cellules pluripotentes sur un substrat insoluble présentant un peptide qui se lie aux glycosaminoglycanes, comme l'héparine. Spécifiquement, elle concerne des procédés pour faire croître et maintenir des cellules pluripotentes sur des substrats ayant une surface chimiquement définie présentant au moins un peptide contenant des résidus aminoacides basiques séparés par un ou deux résidus aminoacides hydrophobes.

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