C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01)
Patent
CA 2690510
A method and oligonucleotides for targeted nucleotide exchange of a duplex DNA sequence, wherein the donor oligonucleotide contains at least two modified nucleotides, at least one of which is a propynylated purine and/or pyrimidine and at least one of which is a LNA having a higher binding affinity compared to naturally occurring A, C, T or G and/or binds stronger to a nucleotide in an opposite position in the first DNA sequence as compared to a naturally occurring nucleotide complementary to the nucleotide in the opposite position in the first DNA sequence.
L'invention porte sur un procédé et sur des oligonucléotides permettant l'échange nucléotidique ciblé d'une séquence d'ADN double brin, l'oligonucléotide donneur contenant au moins deux nucléotides modifiés, dont l'un au moins est une purine propynylée et/ou une pyrimidine et dont l'autre au moins est un acide nucléique verrouillé possédant une affinité de liaison supérieure à celle d'un A, C, T ou G naturel et/ou qui se lie de manière plus forte à un nucléotide en position opposée dans la première séquence d'ADN qu'un nucléotide naturel complémentaire du nucléotide situé en position opposée dans la première séquence d'ADN.
Keygene N.v.
Ridout & Maybee Llp
LandOfFree
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