Treatment of tumor cells for use in immunotherapy of cancer

Details Treatment of tumor cells for use in immunotherapy of cancer Treatment of tumor cells for use in immunotherapy of cancer

A - Human Necessities

61

K

A61K 39/00 (2006.01) C12N 5/09 (2010.01) A01N 1/00 (2006.01) A61K 35/12 (2006.01) A61K 47/10 (2006.01) A61P 35/00 (2006.01) C12N 1/04 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2474954

A method comprising exposing tumor cells to ethanol has been found to preserve the tumor cells during storage. As compared to control cells, tumor cells are preserved for a longer time, and retain display of antigen. In a specific embodiment, modified or unmodified cells are exposed to a concentration of about 37.5% (v/v) ethanol for a period of about 10 minutes at about 4 ~C. Methods of storing haptenized tumor cells and vaccine preparations are also provided. It has also been found that tumor cell vaccines which comprise mainly dead or non-Trypan Blue-excluding cells can have retained or even improved antigenicity as compared to live cells. Methods of preparing and using such vaccines are also described.

L'invention concerne un procédé selon lequel l'exposition de cellules tumorales à l'éthanol permet de les conserver pendant le stockage. Par rapport aux cellules témoins, les cellules tumorales se conservent plus longtemps et l'apparition d'antigènes est préservée. Dans un mode de réalisation spécifique, des cellules modifiées ou non modifiées sont exposées pendant au moins 10 minutes à une concentration d'éthanol d'environ 37,5 % (v/v), à environ 4 ·C. Par ailleurs, l'invention concerne des procédés de stockage de cellules tumorales hapténisées ainsi que des préparations de vaccins. De plus, il a été déterminé que les vaccins à base de cellules tumorales comportant principalement des cellules mortes ou des cellules de non exclusion du bleu de trypan peuvent conserver, voire même améliorer, leur pouvoir antigénique par rapport aux cellules vivantes. L'invention concerne enfin des procédés de préparation et d'utilisation de ces vaccins.

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