Recombinant calf intestinal alkaline phosphatase

Details Recombinant calf intestinal alkaline phosphatase Recombinant calf intestinal alkaline phosphatase

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 9/16 (2006.01) C12N 15/11 (2006.01) G01N 33/535 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2102294

2102294 9318139 PCTABS00025 The invention relates to isolated nucleic acids encoding recombinant calf intestinal alkaline phosphatase. Expression vectors and host cells transformed or transfected with such vectors are also provided. The invention further provides multifunctional polypeptides containing amino acid sequences encoding for calf intestinal alkaline phosphatase and a second amino acid sequence encoding a reagent having specific reactivity with a ligand. The recombinant calf intestinal alkaline phosphatase or its active fragments and the multifunctional polypeptides can be used in the methods for determining the presence or concentration of a ligand.

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