Method and a kit for the detection of adenoviruses

Details Method and a kit for the detection of adenoviruses Method and a kit for the detection of adenoviruses

C - Chemistry, Metallurgy

12

N

C12N 15/34 (2006.01) A61K 39/235 (2006.01) C07K 14/075 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2549124

Method for the simultaneous detection of adenoviruses of sub- genera A, B, C, D, E and F in a sample comprising the steps: a) ~providing a sample, b) ~contacting said sample with at least one forward primer and at least one reverse primer having at least 80% identity with a portion of a nucleotide sequence of a hexon gene of an adenovirus of subgenera A, B, C, D, E and F and be- ing derived from sequences SEQ ID No. 1 and/or SEQ ID No. 2 and/or SEQ ID No. 3 and/or SEQ ID No. 4 to 8 and/or SEQ ID No. to 12 and preferably at least one forward primer and at least one reverse primer having at least 80% identity with a portion of a fiber gene of at least one adenovirus of subgenera A, B, C, D, E and/or F, c) ~subjecting the sample contacted with said primers to a nucleic acid amplification technique, and d) ~optionally determining the presence of adenoviruses in said sample by detecting a nucleic acid amplification prod- uct.

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