Synuclein-based assay and novel synuclein protein

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A01K 67/027 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2244880

A novel member of the synuclein family has been identified and is termed persyn. The full amino acid sequence for the mouse and human isoforms are given in SEQ ID Nos 2 and 4, with the respective nucleotide coding sequences being given as SEQ ID Nos 1 and 3. The unusual expression pattern noted for persyn, in neurons and in skin, led to the realisation that synucleins affect intracellular intermediate filament structure. Thus, an assay for cells exhibiting abnormal intermediate filament structure is described, based upon evaluation of intracellular synuclein. The assay is special relevance to neurodegenerative disorders characterised by plaque deposition, to cancers (especially breast cancer) and to skin disorders. An assay for screening agents of potential therapeutic value is also described.

Un nouveau membre de la famille des synucléines a été découvert et désigné sous le nom de persyne. La séquence entière d'acides aminés pour les isoformes murine et humaine est donnée dans les SEQ ID nos 2 et 4, les séquences de codage respectives des nucléotides étant SEQ ID nos 1 et 3. Le modèle d'expression inhabituel observé pour la persyne, dans les neurones et dans la peau, a mené à la conclusion que les synucléines affectent la structure des filaments intermédiaires intracellulaires. Aussi, décrit-on une épreuve pour les cellules affichant une structure de filaments intermédiaires anormale, basée sur l'évaluation des synucléines intracellulaires. L'épreuve est particulièrement pertinente pour les maladies neurodégénératives caractérisées par le dépôt de plaques, les cancers (en particulier le cancer du sein) et les troubles dermatologiques. On décrit en outre une épreuve pour le criblage d'agents ayant une valeur thérapeutique potentielle.

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