Methods of concentrating microorganisms using affinity...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/24 (2006.01) B01D 39/16 (2006.01) C12M 1/12 (2006.01) C12N 1/02 (2006.01) G01N 1/40 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2347004

A method for concentrating a particular microorganism or microorganisms of interest in a sample is provided and effected by contacting the sample with a matrix to which is bound an affinity receptor specific for the particular microorganism or microorganisms, the affinity receptor and the matrix being selected so as to allow capture of the microorganism or microorganisms to the matrix via the affinity receptor when present in the sample at a concentration of ultra low levels, thereby obviating the need for a prolonged pre-enrichment step, and in some cases obviating altogether the need for a pre-enrichment step, of the microorganism or microorganisms in the sample.

L'invention concerne un procédé permettant de concentrer un(des) micro-organisme(s) particulier(s) ou d'intérêt dans un échantillon. Pour ce faire, on met l'échantillon en contact avec une matrice à laquelle est lié un récepteur d'affinité spécifique au(x) micro-organisme(s) particulier(s), le récepteur d'affinité et la matrice étant sélectionnés de manière à permettre la capture du(des) micro-organisme(s) vers la matrice au moyen du récepteur d'affinité, lorsque le(s) micro-organisme(s) est(sont) présent(s) en quantité négligeable dans l'échantillon. Aussi, l'étape de pré-enrichissement prolongée est-elle nécessaire et dans certains cas, il faut la combiner avec une étape de pré-enrichissement du(des) micro-organisme(s) dans l'échantillon.

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