G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/58 (2006.01) C12Q 1/28 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01)
Patent
CA 2599197
A method of assessing the in vivo state of phagocytes in a patient, possibly indicating diagnostically important states such as inflammation or infection, which method utilizes chemiluminescent (CL) light emitted during the reaction in vitro between a CL substrate and the reactive oxygen species (ROS) formed in a fluid sample obtained from the patient. The measurement is performed in two or more portions of the sample, with stimulated phagocytes affected by one or more priming agents which shift the functional state of the phagocytes, providing a plurality of measurements, which are analyzed so as to distinguish intracellular and extracellular contributions to the CL kinetics. The results are compared with a range of control measurements performed with patients suffering from various diagnostic conditions.
La présente invention concerne un procédé d~évaluation de l~état in vivo de phagocytes chez un patient, pouvant être indicateur d~états importants sur le plan diagnostique, tels que l~inflammation ou l~infection; cette méthode fait appel à la chimioluminescence émise au cours de la réaction survenant in vitro entre un substrat de chimioluminescence et l~espèce réactive d~oxygène dans un échantillon de liquide obtenu auprès du patient. La mesure est effectuée dans deux portions ou plus de l~échantillon, les phagocytes stimulés étant affectés par un ou plusieurs agents d~amorçage qui modifient leur état fonctionnel ; il en résulte plusieurs mesures, lesquelles sont analysées afin de distinguer l~apport intracellulaire et l~apport extracellulaire à la cinétique de la chimioluminescence. Ces résultats sont comparés à une série de mesures témoins réalisées chez des patients souffrant de divers états diagnostiques.
Magrisso Moni
Marks Robert S.
Ben Gurion University Of The Negev Research And Development Auth
Goudreau Gage Dubuc
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