A method for producing a population of homozygous stem cells...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0735 (2010.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01) C12Q 1/68 (2006.01) C40B 40/02 (2006.01) C40B 50/06 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2433419

A method of producing a homogenous population of homozygous stem (HS) cells pre-selected for immunotype and/or genotype from donor cells is described herein. The invention relates to methods of using immunohistocompatible HS cells for diagnosis, therapeutic and cosmetic transplantation, and the treatment of various genetic diseases, neurodegenerative diseases, traumatic injuries and cancer. The invention further relates to methods for using histocompatible HS stem cells pre-selected for a non-disease genotype for prophylactic and therapeutic intervention including, but not limited to, therapeutic and cosmetic transplantation, and the treatment of various genetic diseases, neurodegenerative diseases, and cancer. Furthermore, the invention relates to a catalogued transplant depository of HS cells derived from multiple donors, each of the HS cells being homozygous for a unique HLA haplotype, for the purpose of having a constant, reliable, comprehensive supply of immunohistocompatible cells for diagnosis, treatment and/or transplantation.

L'invention concerne un procédé permettant de produire une population homogène de cellules souches homozygotes (HS) dont l'immunotype et/ou le génotype sont présélectionnés à partir de cellules donatrices. L'invention concerne également des procédés d'utilisation de cellules souches homozygotes immuno-histocompatibles à des fins de diagnostic, de traitement et de transplantation cosmétique et à des fins de traitement de diverses maladies génétiques, maladies neurodégénératives, lésions traumatiques et du cancer. L'invention concerne en outre des procédés permettant d'utiliser des cellules souches homozygotes immuno-histocompatibles présélectionnées pour un génotype non pathologique afin de pratiquer des interventions prophylactiques et thérapeutiques comprenant, entre autres, une transplantation thérapeutique et cosmétique et afin de traiter diverses maladies génétiques, maladies neurodégénératives ainsi que le cancer. L'invention concerne enfin une banque cataloguée de transplantation constituée de cellules souches homozygotes provenant de multiples donneurs, chacune des cellules souches étant homozygote pour un seul haplotype HLA. Cette banque permet de disposer d'une réserve importante, fiable et constante de cellules à des fins de diagnostic, de traitement et/ou de transplantation.

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