Recombinant stable cell clone, its production and use thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) C07K 14/745 (2006.01) C07K 14/755 (2006.01) C12N 5/00 (2006.01) C12N 9/64 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2381173

The invention relates to a stabile recombinant cell clone which is stabile in a medium containing no serum and proteins for at least 40 generations, and to a biomass which is obtained by multiplying the stabile cell clone under cultivation conditions that do not involve the use of serum or proteins. The invention also relates to a method for producing recombinant proteins using the biomass, to a method for producing stabile recombinant cell clones, and to the production of a recombinant protein in a synthetic minimal medium that does contain serum or proteins.

La présente invention concerne: un clone cellulaire recombiné stable pour au moins 40 générations dans un milieu dépourvu de sérum et de protéines; une biomasse obtenue par multiplication du clone dans des conditions de culture dépourvues de sérum et de protéines; et un procédé de production de protéines recombinées grâce à la biomasse. Cette invention concerne également un procédé de production de clones recombinés stables et la production d'une protéine recombinée dans milieu minimum de synthèse dépourvu de sérum et de protéines.

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