Detection of intact recombinant viruses

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/70 (2006.01) C12N 7/02 (2006.01) C12Q 1/68 (2006.01) G01N 30/96 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2579478

In accordance with the present invention, there is provided a new method for detection and quantification of virus and viral particles, comprising the step of labeling the nucleic acids of an intact virus or viral particle with a dye that emits fluorescence once complexed with the nucleic acids and detecting the virus or viral particle with a chromatograph equipped with a fluorescence detector.

La présente invention concerne un nouveau procédé pour détecter et quantifier des virus et des particules virales. Ce procédé consiste à marquer les acides nucléiques d'un virus intact ou de particules virales intactes avec un colorant qui émet une fluorescence une fois qu'il est complexé avec lesdits acides nucléiques, puis à détecter le virus ou les particules virales avec un chromatographe équipé d'un détecteur de fluorescence.

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