Generation of artificial binding proteins based on ubiquitin...

Details Generation of artificial binding proteins based on ubiquitin... Generation of artificial binding proteins based on ubiquitin...

C - Chemistry, Metallurgy

07

K

C07K 14/435 (2006.01) C07K 14/47 (2006.01) C12N 15/09 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2524899

The invention relates to modified proteins of the superfamily of "ubiquitin- like proteins", proteins that have a ubiquitin-like fold and fragments or fusion proteins thereof. As a result of said modification, the proteins have a binding affinity with respect to a predetermined binding partner that did not exist previously. The invention also relates to a method for the production and utilization of said proteins.

L'invention concerne des protéines modifiées de la superfamille des protéines apparentées à l'ubiquitine, des protéines présentant un motif de repliement apparenté à celui de l'ubiquitine, ainsi que des fragments ou des protéines de fusion desdites protéines. Selon l'invention, la modification de ces protéines entraîne chez elles une affinité de liaison jusqu'alors inexistante envers un élément de liaison correspondant prédéterminé. Cette invention se rapporte en outre à un procédé de production desdites protéines, ainsi qu'à leur utilisation.

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