Method for isolating nucleic acids and protein from a single...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2473376

The present invention comprises a method of isolating nucleic acid and protein from the same sample with solid supports, wherein nucleic acid and protein components contained in the sample become bound to distinct solid supports. The invention also allows for kits for isolating nucleic acid and protein from the same sample and for use of the method of isolating nucleic acid and protein for the analysis and/or comparison of mRNA and/or protein expression and/or their correlation to genomic information.

La présente invention se rapporte à une méthode qui permet d'isoler, avec des supports solides, de l'acide nucléique et des protéines contenus dans le même échantillon, les constituants d'acide nucléique et de protéine contenus dans l'échantillon se liant à des supports solides distincts. Cette invention concerne également des trousses permettant d'isoler de l'acide nucléique et des protéines contenus dans le même échantillon et l'utilisation de ladite méthode pour isoler de l'acide nucléique et des protéines afin d'analyser et/ou de comparer l'expression de l'ARN messager et/ou des protéines et/ou d'effectuer leur corrélation avec les informations génomiques.

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