Recombinant cells, dna constructs, vectors and methods for...

Details Recombinant cells, dna constructs, vectors and methods for... Recombinant cells, dna constructs, vectors and methods for...

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) A23K 1/165 (2006.01) C12N 1/15 (2006.01) C12N 9/16 (2006.01) C12N 15/80 (2006.01)

Patent

CA 2141431

2141431 9403072 PCTABS00030 A recombinant combination strain which is capable of over-expressing at least two different genes under two separate promoters in filamentous fungi. The genes encode phytase and pH 2.5 acid phosphatase. GA- and GAPDH-promoters are preferably used. Mixtures containing desired ratios of the two enzymes are prepared by recombinant DNA techniques. The enzyme mixture shows a cooperative effect in the degradation of phytic acid and its salts. Preferred ratios of the two enzymes are about 3:1 to about 16:1. The DNA sequences were isolated from a wild-type Aspergillus niger ALKO243 (ATCC#38854).

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