Screening method for compounds active in treating myopia and...

A - Human Necessities – 61 – K

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A61K 31/522 (2006.01) A61K 31/00 (2006.01) A61K 31/52 (2006.01) A61P 27/02 (2006.01)

Patent

CA 2276287

The invention relates to a method for identification of substances which are applicable for treatment or prevention of an insufficient longitudinal growth of the eye (hypermetropia) or for treatment or prevention of an excessive longitudinal growth of the eye (myopia); substances identified by the method for treating or preventing conditions related to the longitudinal growth of the eye; substances and mixtures of substances for the preparation of a pharmaceutical composition for the treatment or prevention of abnormal growth of the axial length of the eye. The identification involves measuring the effect of the substances on the retinal pigment epithelium of the eye, e.g. by detecting the metabolic effect of the substance on the retinal epithelium, the effect on the standing potential or the effect on the proteoglycanes of the scleral tissue of the eye, by way of EOG examination, by way on the size of the so-called c-wave in ERG-recordings, or by the state of the Ca2+-channels or on the [3H]-ryanodine receptors of the retinal pigment epithelium.

Procédé pour identifier des substances pouvant être utilisées pour le traitement ou la prévention d'une croissance longitudinale insuffisante de l'oeil (hypermétropie) ou pour le traitement ou la prévention d'une croissance longitudinale excessive de l'oeil (myopie). L'invention porte également sur des substances identifiées par ce procédé, pour le traitement et la prévention de troubles liés à la croissance longitudinale de l'oeil, et sur des substances et mélanges de substances servant à préparer une composition pharmaceutique pour le traitement ou la prévention d'une croissance anormale de la longueur axiale de l'oeil. L'identification consiste à mesurer l'effet des substances sur l'épithélium pigmentaire de la rétine, p. ex. en détectant l'effet métabolique de la substance sur cet épithélium, l'effet sur le potentiel stationnaire ou l'effet sur les protéoglycanes du tissu scléral de l'oeil, au moyen d'une électro-occulographie, en mesurant l'amplitude de "l'onde c" dans les électrorétinogrammes ou en se référant à l'état des canaux de Ca<2+> ou des récepteurs de [<3>H]-ryanodine de l'épithélium pigmentaire de la rétine.

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