Bifunctional cosmid useful for cloning actinomycete dna

Details Bifunctional cosmid useful for cloning actinomycete dna Bifunctional cosmid useful for cloning actinomycete dna

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C12N 15/52 (2006.01) C12N 15/74 (2006.01) C12N 15/76 (2006.01) C12P 29/00 (2006.01) C12Q 1/18 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2047837

31,254-00 A BIFUNCTIONAL COSMID USEFUL FOR CLONING ACTINOMYCETE DNA ABSTRACT OF THE DISCLOSURE The present invention relates to novel and useful cosmids for cloning and expressing the isolated DNA gene cluster which directs the synthesis of tetracycline and chlortetracycline, in a heterologous host such as Streptomyces lividans. The claimed plasmids (cosmids) are isolated by screening a cosmid library, constructed in Escherichia coli for the expression of tetracycline resistance in S. lividans. Placids LP2127 and LP2128 are recovered from such tetracycline-resistant S. lividans isolates shown by HPLC analysis of agar and broth fermentations to direct the synthesis of tetracycline and chlortetracycline.

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