Determining alcohol intake using sialic acid/apo j

G - Physics – 01 – N

Patent

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G01N 33/50 (2006.01) G01N 33/66 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2301634

Disclosed is a method for determining alcohol intake or alcohol liver damage in a subject by quantifying the content of sialic acid in apolipoprotein J (Apo J). In particular the present invention involves the steps of (a) providing a sample containing Apo J from the subject; (b) purifying the Apo J from the sample; (c) determining sialylation index, i.e. moles sialic acid per mole of Apo J in the sample; and evaluating whether the sialylation index is an indication of alcohol intake or alcohol related liver damage recovery from alcohol addiction or alcohol relapse in the subject.

L'invention concerne un procédé permettant de déterminer la consommation d'alcool ou les atteintes hépatiques liées à l'alcool chez un sujet. Le procédé consiste à quantifier le contenu d'acide sialique dans l'apolipoprotéine J (Apo J). Ce procédé consiste plus particulièrement à a) apporter un échantillon comportant de l'Apo J prélevé du sujet; b) à purifier l'Apo J à partir de l'échantillon; c) à déterminer l'indice de sialylation, notamment les moles d'acide sialique par mole d'Apo J se trouvant dans l'échantillon; et à déterminer en fonction de l'indice de sialylation si le sujet consomme de l'alcool ou s'il souffre d'atteintes hépatiques. On peut aussi déterminer en fonction de l'indice de sialylation le degré de rétablissement ou encore les rechutes du sujet.

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