Recombinant dna coding for a novel protein having .beta.-1,3...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) A01H 5/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2083837

The invention relates to a novel recombinant DNA coding for a protein having .beta.-1,3-glucanase activity or a precursor thereof comprising the following amino acid sequence (a1): Ile Gly Val Cys Tyr Gly Met Leu Gly Asn Asn Leu Pro Ser Ala Asn Asp Val Ile Gly Leu Tyr Arg Ser Asn Asn Ile Lys Arg Met Arg Leu Tyr Asp Pro Asn Gln Ala Ala Leu Glu Ala Leu Arg Asn Ser Gly Ile Glu Leu Ile Leu Gly Val Pro Asn Ser Asp Leu Gln Gly Leu Ala Thr Asn Pro Asp Thr Ser Arg Gln Trp Val Gln Lys Asn Val Leu Asn Phe Trp Pro Ser Val Lys Ile Lys Tyr Val Ala Val Gly Asn Glu Val Ser Pro Val Gly Gly Ser Ser Ser Val Ala Gln Tyr Val Leu Pro Ala Ile Gln Asn Val Tyr Gln Ala Ile Arg Ala Gln Gly Leu His Asp Gln Ile Lys Val Ser Thr Ser Ile Asp Met Thr Leu Ile Gly Asn Ser Phe Pro Pro Ser Gln Gly Ser Phe Arg Gly Asp Val Arg Ser Tyr Leu Asp Pro Ile Ile Gly Tyr Leu Val Tyr Ala Asn Ala Pro Leu Leu Val Asn Val Tyr Pro Tyr Phe Ser Tyr Thr Gly Asn Pro Arg Asp Ile Ser Leu Pro Tyr Ala Leu Phe Thr Ala Pro Asn Val Val Val Trp Asp Gly Gln Tyr Gly Tyr Gln Asn Leu Phe Asp Ala Met Leu Asp Ser Val His Ala Ala Ile Asp Asn Thr Lys Ile Gly Tyr Val Glu Val Val Val Ser Glu Ser Gly Trp Pro Ser Asp Gly Gly Phe Ala Ala Thr Tyr Asp Asn Ala Arg Val Tyr Leu Asp Asn Leu Val Arg Arg Ala Asn Arg Gly Ser Pro Arg Arg Pro Ser Lys Pro Thr Glu Thr Tyr Ile Phe Ala Met Phe Asp Glu Asn Gln Lys Asn Pro Glu Ile Glu Lys His Phe Gly Leu Phe Asn Pro Asn Lys Gln Lys Lys or a sequence possessing a high degree of homology with the sequence (a1).

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