A porin gene from campylobacter jejuni, related products and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) A61K 39/106 (2006.01) C07K 14/205 (2006.01) C07K 16/12 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2284844

The invention relates to a porin gene from Campylobacter jejuni [SEQ ID NO:3]. The gene has been designated porA and is 1275 bp in length and expresses a protein of 45.6 kDa having a pI of 4.44 [SEQ ID NO:2]. The sequencing and cloning of the gene makes possible various medical and industrial uses. For examples, knowledge of the DNA code makes it possible to design DNA probes for identification of the gene in samples for testing. A positive result indicates the presence of the gene in the sample and is a strong indicator of the presence of C. jejuni. Such probes can also be used to isolate the corresponding cDNA, that may then be amplified by polymerase chain reaction. The development of DNA probes based on a known sequence is a known procedure that is familiar to persons skilled in the art and it will be possible for such persons to develop suitable probes without undue experimentation. Normally, such probes would consist of at least 15 consecutive nucleotides from the cDNA sequence.

L'invention concerne un gène de porine extrait de Campylobacter jejuni (SEQ ID NO:3). Ce gène, dénommé porA, a une longueur de 1275 bp et exprime une protéine de 45,6 kDa ayant un pHi de 4,44 (SEQ ID NO:2). Le séquençage et le clonage du gène autorise diverses utilisations médicales et industrielles. Par exemple, la connaissance du code de l'ADN permet de concevoir des sondes d'ADN destinées à l'identification du gène dans des échantillons à des fins d'analyse. Un résultat positif indique la présence du gène dans l'échantillon et constitue un puissant indicateur de la présence de C. jejuni. Ces sondes peuvent également être utilisées pour isoler l'ADNc correspondant, qui peut ensuite être amplifié par PCR. La mise au point des sondes d'ADN sur la base d'une séquence connue est une technique connue, familière aux personnes versées en la matière, et elle leur permettra de mettre au point des sondes appropriées sans expérimentation inutile. Normalement, ces sondes devraient comprendre au moins 15 nucléotides consécutifs de la séquence d'ADNc.

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