Methods for detecting and reversing resistance to...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A01N 43/90 (2006.01) A61K 31/365 (2006.01) C07K 14/435 (2006.01) C07K 16/18 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2288853

This invention describes nucleic acid molecules or the fragments thereof, extracted from nematode or arthropod pests or recombinant, which encode P- glycoprotein homologs and regulate resistance to the macrocyclic lactone compounds, and their expression product. Also described are methods for detecting the gene encoding for resistance to the macrocyclic lactone compounds in nematode or arthropod pests which comprise comparing nucleic acids extracted from a pest specimen to those encoding for resistance and susceptibility to the pesticides. The invention is further drawn to methods and compositions for increasing the efficacy of the macrocyclic lactone compounds against resistant nematode or resistant arthropod pests which comprise administering to a mammal or applying to crops an effective amount of a multidrug resistance reversing agent.

Cette invention concerne des molécules d'acide nucléique, ou des fragments de celles-ci, qui sont extraits de nématodes ou d'arthropodes parasites ou d'un recombinant. Ces molécules codent les homologues de P-glycoprotéines et permettent de réguler la résistance aux composés de lactone macrocycliques. Outre les produits d'expression de ces molécules, cette invention concerne également des procédés de détection du gène codant pour la résistance aux composés de lactone macrocycliques chez nématodes ou arthropodes parasites. Ces procédés consistent à comparer les acides nucléiques extraits d'un spécimen de parasite à ceux codant pour la résistance et la sensibilité aux pesticides. Cette invention concerne en outre des procédés et des compositions permettant d'accroître l'efficacité des composés de lactones macrocycliques contre des nématodes ou des arthropodes parasites résistants, ces procédés consistant à administrer à un mammifère ou à appliquer sur des cultures une quantité efficace d'un agent d'inversion de résistance aux médicaments polyvalents.

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