High-throughput methods of screening dna for deletions and...

Details High-throughput methods of screening dna for deletions and... High-throughput methods of screening dna for deletions and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) A01H 1/00 (2006.01) A01H 5/10 (2006.01)

Patent

CA 2526533

The subject invention relates to high-throughput methods of screening DNA for mutations. These methods offer various unexpected advantages over current methods. In a preferred embodiment, the subject invention includes pooling DNA samples from many plants that were subjected to mutagenesis. Methods of the subject invention include highly sensitive means for detecting individual mutants, preferably deletions, in large pools or collections of DNA samples.

L'invention concerne des procédés de criblage à haut débit à des fins de mutations. Ces procédés offrent divers avantages inattendus par rapport aux procédés actuels. Dans un mode de réalisation préféré, l'invention consiste à mélanger des échantillons d'ADN provenant de plusieurs plantes ayant été soumises à la mutagenèse. Les procédés de l'invention font intervenir des moyens à haute sensibilité pour détecter des mutants individuels, de préférence, des délétions, dans de grands pools ou ensembles d'échantillons d'ADN.

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