C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/70 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/13 (2006.01) C12N 15/63 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C40B 40/02 (2006.01) C40B 40/10 (2006.01) C40B 50/06 (2006.01) C07K 1/04 (2006.01) C40B 40/08 (2006.01)
Patent
CA 2458462
Methods useful in constructing libraries that collectively display and/or express members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens.
La présente invention concerne des procédés utiles dans la construction de bibliothèques qui de manière collective affichent et/ou expriment des membres de diverses familles de peptides, polypeptides ou protéines et les bibliothèques produites en mettant en oeuvre lesdits procédés. L'invention concerne également des procédés de criblage desdites bibliothèques et des peptides, polypeptides ou protéines identifiés par de tels procédés de criblage. FIG. 1 : 1 AMPLIFICATION DE GENES DE CHAINE LOURDE SANS UTILISATION DE SEQUENCES SPECIFIQUES AUX GENES DE CHAINE LOURDE A SIGNAL UTR ; VARIABLE ; CONSTANT ; UTR AA A ; ARNm B TRANSCRIPTION INVERSE ; CCC ; BRIN ADN INFERIEUR PROLONGE PAR ARNm C TRANSCRIPTION INVERSE ; GGG ; CCC BRIN INFERIEUR ADN PROLONGE PAR LE COMPLEMENT INVERSE D REACTION EN CHAINE PAR LA POLYMERASE ; GGG ; CCC ; ADN A DOUBLE-BRIN E CAPTURE SUR BILLES DE STREPTAVIDINE ; GGG ; CCC ; ADNc A DOUBLE BRIN SUSCEPTIBLE D'ETRE IMMOBILISE
Cohen Edward H.
Hoet Rene
Hoogenboom Hendricus R. J. M.
Ladner Robert C.
Nastri Horacio G.
Dyax Corp.
Smart & Biggar
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