C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 7/64 (2006.01) A23D 9/00 (2006.01) A23D 9/02 (2006.01) A23K 1/16 (2006.01) A23L 1/30 (2006.01) A61K 8/92 (2006.01) A61K 31/20 (2006.01) A61K 31/232 (2006.01) C11B 1/10 (2006.01) C11C 1/00 (2006.01) C12N 1/00 (2006.01)
Patent
CA 2489982
The present invention provides a process for the production of a microbial oil comprising culturing a micro-organism in a two stage fermentation process where, in a last stage that precedes the end of fermentation, the carbon source is: consumed by the micro-organisms at a rate greater than it is added to the medium; added at a rate <= 0.30 M carbon/kg medium; or is rate limiting on the growth of the micro-organism. The micro-organisms thus have the carbon source restricted so that they preferentially metabolise fats or lipids other than arachidonic acid (ARA), so increasing the proportion of ARA in the cells. A microbial oil is then recovered from the micro-organism, using hexane as a solvent, that has at least 50% ARA and at least 90% triglycerides.
la présente invention concerne un procédé d'obtention d'huile microbienne consistant à cultiver un micro-organisme en deux étapes de fermentation. Au cours de la dernière étape précédant la fin de la fermentation, la source de carbone est: consommée par les micro-organismes à un rythme plus rapide que son rythme d'alimentation dans le milieu ; ajoutée à un taux = 0.30 M carbone/kg milieu; ou bien est limitée par la croissance des micro-organismes. Comme la source de carbone est limitée, les micro-organismes métabolisent de préférence des graisses ou des lipides autres que l'acide arachidonique (ARA), ce qui augmente la teneur des cellules en ARA. Au moyen d'hexane comme solvant, on extrait ensuite une huile microbienne des micro-organismes qui contient au moins 50 % d'ARA et au moins 90 % de triglycérides.
Brocken Petrus Joseph Maria
Streekstra Hugo
Dsm Ip Assets B.v.
Smart & Biggar
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