Fourier detection of species migrating in a microchannel

Details Fourier detection of species migrating in a microchannel Fourier detection of species migrating in a microchannel

G - Physics

01

N

G01N 27/453 (2006.01) G01N 21/64 (2006.01) G01N 21/85 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2257895

The present invention provides a microfluidic system (100) for fast, accurate and low cost electrophoretic analysis of materials in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields. Light from periodically spaced regions along a channel (110) in the microfluidic system are received by the photodetector (130). The intensity of light received by the photodetector is modulated by the movement of species bands (123) through the channel under electrophoretic forces. By Fourier analysis, the velocity of each species band (123) is determined and the identification of the species is made by its electrophoretic mobility in the channel (110).

L'invention concerne un système microfluidique (100) permettant une analyse électrophorétique rapide, précise et peu coûteuse de matériaux dans le domaine de la chimie, de la biochimie, de la biotechnologie et de la biologie moléculaire, ainsi que dans de nombreux autres domaines. La lumière provenant de zones disposées de manière périodique le long d'un canal (110) du système microfluidique est reçue par un photodétecteur (130). L'intensité de la lumière reçue est modulée par le déplacement des bandes d'espèces chimiques (123) à l'intérieur du canal sous l'effet des forces électrophorétiques. Par une analyse de Fourier, on détermine la vitesse de chaque bande (123) et on identifie l'espèce par sa mobilité électrophorétique dans le canal (110).

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