Laser scanning cytometry mediated analysis of therapeutic...

G - Physics – 01 – N

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G01N 33/50 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2428062

This invention describes the use of a laser scanning device, for example a laser scanning CYTOMETRY (LSC), with a double-fluorescent labeling technique as a quantitative method that can be used to objectively and accurately measure endothelial cell death, endothelial tumor cell death and blood vessel density of tumor tissue. These parameters can be used as markers of efficacy in tumors treated with anti-angiogenic or traditional therapies and can distinguish patients who respond to these drugs from those who do not.

L'invention concerne l'utilisation d'un dispositif à balayage laser, par exemple, une cytométrie à balayage laser, ainsi qu'une technique de marquage à double fluorescence comme procédé quantitatif pouvant s'utiliser afin de mesurer objectivement et précisément la mort des cellules endothéliales, la mort de cellules tumorales endothéliales et la densité des vaisseaux sanguins des tissus tumoraux. Ces paramètres peuvent être utilisés comme marqueurs d'efficacité dans des tumeurs traitées par des thérapies angiogéniques ou traditionnelles et peuvent distinguer les patients qui répondent à ces médicaments des autres.

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