Methods for introducing genes into mammalian subjects

Details Methods for introducing genes into mammalian subjects Methods for introducing genes into mammalian subjects

A - Human Necessities

61

K

A61K 48/00 (2006.01) A61K 35/12 (2006.01) A61K 35/36 (2006.01) C12N 15/00 (2006.01) C12N 15/87 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2383001

Methods to obtain genetic modifications of cells in histoculture are described. Modification is assisted by treating the histoculture with collagenase prior to contacting the histoculture with the delivery vehicle for the desired gene. Hair follicles and other organized tissues can be modified in this way and then transplanted into intact recipients.

L'invention concerne des procédés permettant d'obtenir des modifications génétiques de cellules en culture tissulaire. On facilite ces modifications en traitant la culture tissulaire avec de la collagénase avant de la mettre en contact avec le vecteur du gène désiré. Les follicules pileux et d'autre tissus organisés peuvent être modifiés de cette manière puis transplantés dans des cellules réceptrices intactes.

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