Improved method and composition for determining resistance...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/527 (2006.01) C12Q 1/26 (2006.01) C12Q 1/48 (2006.01) C12Q 1/533 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2456099

The invention describes a method for determining whether a plant is resistant to an acetohydroxyacid synthase ("AHAS") inhibitor. The method comprises: (a) treating a part taken from the plant with a acetohydroxyacid synthase inhibitor, a ketol-acid reductoisomerase inhibitor and an external supplement that is either a compound or combination of compounds selected from the group consisting of alanine, ammonium hydroxide, asparagine, and pyruvic acid or a salt thereof; and (b) measuring the amounts of acetohydroxybutyrate and/or acetolactate.

L'invention concerne un procédé servant à déterminer si une plante est résistante à un inhibiteur de l'acétohydroxyacide synthase ("AHAS"). Le procédé selon l'invention comprend les étapes suivantes : (a) traitement d'une partie prélevée sur la plante avec un inhibiteur de l'acétohydroxyacide synthase, un inhibiteur de la cétol-acide réductoisomérase, ainsi qu'un complément externe qui est un composé ou une combinaison de composés sélectionnés dans le groupe formé par alanine, hydroxyde d'ammonium, asparagine et acide pyruvique ou son sel et (b) mesure des quantités d'acétohydroxybutyrate ou d'acétolactate.

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