Pluripotent stem cell derived from cardiac tissue

Details Pluripotent stem cell derived from cardiac tissue Pluripotent stem cell derived from cardiac tissue

C - Chemistry, Metallurgy

12

N

C12N 5/0775 (2010.01) A61K 35/34 (2006.01) A61P 9/00 (2006.01)

Patent

CA 2600653

Disclosed is a stem cell applicable to a regenerative therapy. Also disclosed is a technique for regenerative therapy using the cell. A piece of a cardiac tissue removed is treated with an enzyme to produce a cell suspension. The cells suspension is then subjected to any one of the following procedures (1) to (3) to produce pluripotent cells: (1) isolation of cells by density gradient method; (2) suspension culture in a medium supplemented with a fibroblast growth factor and an epithelial cell growth factor; and (3) selective isolation of cells forming floating spheres. The pluripotent cells can be used to perform a regenerative therapy.

L~invention concerne une cellule souche applicable à une thérapie régénérative. Elle porte également sur une technique destinée à une thérapie régénérative utilisant la cellule. On traite un morceau de tissu cardiaque avec une enzyme pour produire une suspension de cellules. On soumet alors la suspension de cellules à l~une des procédures suivantes (1) à (3) pour produire des cellules pluripotentes : (1) isolation de cellules selon un procédé de gradient de densité ; (2) culture en suspension dans un milieu complété d~un facteur de croissance de fibroblaste et un facteur de croissance cellulaire épithéliale ; et (3) isolation sélective de cellules formant des sphères flottantes. On peut utiliser les cellules pluripotentes dans le cadre d'une thérapie régénérative.

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