Polypeptide quantitation

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/00 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2468161

The invention relates to methods and materials useful for determining the actual amount of a selected polypeptide in a sample, by measuring the amount of a cleavage product released from the selected polypeptide and using an exogenous polypeptide corresponding to the cleavage product as a standard. These methods and materials can be used, for example, to quantify the actual amount of one or more selected polypeptides in complex samples.

L'invention concerne des procédés et des matériaux utiles pour la détermination de la quantité ponctuelle d'un polypeptide sélectionné dans un échantillon, par mesure de la quantité d'un produit de clivage libéré dudit peptide sélectionné et au moyen d'un polypeptide exogène correspondant au produit de clivage en tant que modèle. Lesdits procédés et matériaux peuvent être utilisés par exemple à des fins de quantification de la quantité ponctuelle d'un ou de plusieurs polypeptides sélectionnés dans des échantillons complexes.

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