C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2362013
Transgenic rice plants obtained by isolating the promoter region of a glutelin gene capable of expressing the gene in the albumen of rice seed; constructing a vector wherein natural and modified soybean glycinin genes are ligated to the downstream of the promoter; and then transferring this vector into cultured rice cells. As the results of examinations on the tissue specificity and the morphology of the soybean glycinin expressed in the transgenic rice plants thus obtained, it is found out that the soybean glycinin expressed in rice is accumulated in rice seeds and the thus accumulated soybean glycinin exists as the processed maturation type.
Selon l'invention, on obtient des plantes de riz transgéniques en isolant la région du promoteur d'un gène de glutéline capable d'exprimer le gène dans l'albumen des graines de riz; en construisant un vecteur dans lequel des gènes de glycinine de soja sont liés à la partie aval du promoteur; enfin, en transférant ce vecteur à des cellules de riz de culture. Les résultats des examens concernant la spécificité tissulaire et la morphologie de la glycinine de soja exprimée dans les plantes de riz transgéniques ainsi obtenues démontrent que la glycinine de soja exprimée dans le riz est accumulée dans les graines de riz et que la glycinine de soja ainsi accumulée existe en tant que type de maturation traité.
Katsube-Tanaka Tomoyuki
Takaiwa Fumio
Utsumi Shigeru
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Bio-Oriented Technology Research Advancement Institution
Japan As Represented By Director General Of Ministry Of Agricult For Estry And Fisheries National Institute Of Agrobiological Res
National Agriculture And Bio-Oriented Research Organization
National Institute Of Agrobiological Sciences
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