C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2087105
This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase. Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction. The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.
Procédés sans danger et efficaces d'extraction d'acides nucléiques. L'invention concerne notamment des procédés d'isolation d'acide nucléique à partir d'un échantillon contenant un mélange biologique d'acides nucléiques et d'autres composés biologiques, dans lesquels l'échantillon est combiné avec une solution d'extraction contenant au moins un composé organique tel que de l'alcool benzoïque ou un dérivé d'alcool benzoïque afin de former une phase aqueuse et une phase non aqueuse. On isole l'acide nucléique de la phase aqueuse. La solution combinée obtenue contient également de préférence une bentonite ayant la notation ci-définie. Typiquement, on combine premièrement l'échantillon avec un agent lytique avant l'extraction. Les agents lytiques préférés sont des sels chaotropes tels que l'hydrochlorure de guanidinium et l'isothiocyanate de guanidinium.
Cimler B. Melina
Meyer Rich B. Jr.
Van Ness Jeffrey
Vermeulen Nicholaas Marthinus Johannes
Becton Dickinson And Company
Fetherstonhaugh & Co.
LandOfFree
Non-corrosive composition and methods useful for the... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Non-corrosive composition and methods useful for the..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Non-corrosive composition and methods useful for the... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1760243