C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/10 (2006.01) C12N 9/22 (2006.01) C12N 15/79 (2006.01) C12N 15/81 (2006.01) C12N 15/82 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01)
Patent
CA 2379115
This invention provides methods for obtaining specific and stable integration of nucleic acids into eukaryotic cells. The invention makes use of site- specific recombination systems that use prokaryotic recombinase polypeptides, such as the .PHI.C31 integrase, that can mediate recombination between the recombination sites, but not between hybrid recombination sites that are formed upon the recombination. Thus, the recombination is irreversible in the absence of additional factors. Eukaryotic cells that contain the recombinase polypeptides, or genes that encode the recombinases, are also provided.
L'invention concerne des procédés permettant d'obtenir une intégration spécifique et stable d'acides nucléiques dans des cellules eucaryotes. En l'occurrence, des systèmes de recombinaison spécifiques au site utilisent des poplypeptides recombinases procaryotes, tels que .PHI.C31 intégrase, qui peuvent induire la recombinaison entre les sites de recombinaison, mais non entre des sites de recombinaison hybrides qui sont formés par suite de la recombinaison. Ainsi, la recombinaison est irréversible en l'absence de facteurs supplémentaires. L'invention traite également de cellules eucaryotes qui renferment des polypeptides recombinases, ou des gènes qui codent les recombinases.
Calendar Richard
Ow David W.
Thomason Lynn
Fetherstonhaugh & Co.
The Regents Of The University Of California
The United States Of America As Represented By The Secretary Of
LandOfFree
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