Novel lactose phosphorylase enzymes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/10 (2006.01)

Patent

CA 2709431

The present invention relates to novel lactose phosphorylase enzymes and the uses thereof. More specifically, the invention relates to lactose phosphorylase enzymes created by mutation of a cellobiose phosphorylase from Cellulomonas uda. By introducing mutations in this enzyme, the activity can be switched from cellobiose phosphorylase into lactose phosphorylase.

La présente invention porte sur de nouvelles enzymes lactose phosphorylase et sur leurs utilisations. Plus spécifiquement, l'invention porte sur des enzymes lactose phosphorylases créées par mutation d'une cellobiose phosphorylase provenant de Cellulomonas uda. L'introduction de mutations dans cette enzyme permet de basculer l'activité enzymatique de cellobiose phosphorylase à lactose phosphorylase.

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