Trimer of hiv env gene expression product

Details Trimer of hiv env gene expression product Trimer of hiv env gene expression product

C - Chemistry, Metallurgy

12

N

C12N 15/49 (2006.01) A61K 39/21 (2006.01) C07K 14/16 (2006.01) G01N 33/68 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2338020

The invention concerns a purified recombinant glycoprotein having the following properties: a) an adherence capacity to CD4; b) an affinity with a anti-gp120 antibody capable of neutralising in vitro HIV cell infection; c) an affinity with an anti-gp41 antibody; d) a trimeric form free from inter-chain disulphide bonds. The invention also concerns a vaccine comprising said purified glycoprotein and an adjuvant. The invention further concerns a method for obtaining said glycoprotein as per Claim 1, which consists in expressing, by means of genetic recombining techniques, a glycoprotein corresponding to the properties a), b) and c) mentioned in Claim 1; purifying it, and subjecting it to steps involving at least a reducing agent, an ionic detergent and/or a neutral detergent in conditions leading to a glycoprotein having said properties.

Glycoprotéine recombinante purifiée répondant aux propriétés suivantes: a) une capacité d'adhésion au CD4; b) une affinité avec un anticorps anti-gp120 capable de neutraliser in vitro l'infection de cellules par HIV; c) une affinité avec un anticorps anti-gp41; d) une forme trimérique dépourvue de ponts disulfures inter-chaînes. Vaccin comprenant la glycoprotéine purifiée selon l'invention et un adjuvant. Procédé d'obtention d'une glycoprotéine selon la revendication 1, dans lequel on exprime, au moyen de techniques de recombinaison génétique, une glycoprotéine répondant aux propriétés a), b) et c) énoncées à la revendication 1, on la purifie, et on la soumet à des étapes impliquant au moins un agent réducteur, un détergent ionique et/ou un détergent neutre dans des conditions telles que l'on obtient une glycoprotéine besoins de l'invention.

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