Polynucleotide comprising sequences of wheat gliadins and...

Details Polynucleotide comprising sequences of wheat gliadins and... Polynucleotide comprising sequences of wheat gliadins and...

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/10 (2006.01)

Patent

CA 2750997

The present invention relates to the specific silencing of the .alpha.(alpha), .beta.(beta), .gamma.(gamma) and .omega. (omega)-gliadins of hard wheat for flour by RNA interference (RNAi) through employment of a polynucleotide which is transcribed into an hpRNA (hairpin RNA). Furthermore the present invention additionally relates to a vector, cell, plant or seed comprising the polynucleotide, the expression whereof is specifically directed in particular tissues of wheat seeds through gene expression--regulating sequences such as, for example, the promoter of a gene of .gamma.-gliadins or the promoter of the gene encoding for a D--hordein.

La présente invention concerne le silençage spécifique des a (alpha), ß (bêta), ? (gamma) et ? (oméga)-gliadines de blé dur et meunier par ARN interférent (ARNi) au moyen de l'utilisation d'un polynucléotide qui est transcrit en un hpARn (ARN en épingle à cheveux). De plus, la présente invention concerne également un vecteur, une cellule, une plante ou une semence qui comprennent le polynucléotide, dont l'expression est dirigée de manière spécifique vers des tissus concrets des semences de blé par l'intermédiaire de séquences régulatrices de l'expression génique comme par exemple, le promoteur d'un gène de ?-gliadines ou le promoteur du gène qui code pour une D-hordéine.

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