Epithelial cell cultures for in vitro testing

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2243130

A method and a medium for culturing epithelial cells of both normal and malignant origin is provided. The method entails physically disaggregating tissue samples, placing the resulting fragments onto a surface comprised of basement membrane matrix components, and culturing the tissue in a medium containing preselected fetal and newborn calf sera and rat sera. Both primary explant cell cultures and cell lines, which are long-lived and particularly suitable for further study, are produced. The cultured primary explant cells undergo differentiation to form complex structures resembling those seen in vivo.

Divulgation d'une méthode et d'un milieu pour cultiver des cellules épithéliales d'origine à la fois normale et maligne. La méthode consiste à désagréger les échantillons de tissu, à placer les fragments obtenus sur une surface d'une matrice formée d'une membrane basale reconstituée et à cultiver le tissu dans un milieu contenant des sérums de foetus de veau et de veaux nouveau-nés et de sérums de rats présélectionnés. On peut ainsi produire des cultures de cellules d'explants primaires et de lignées cellulaires ayant une longue durée de vie et convenant particulièrement pour des études plus approfondies. Les cellules d'explants primaires cultivées subissent une différenciation pour former des structures complexes ressemblant à celles observées in vivo.

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