Multi-sample fermentor and method of using same

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 1/00 (2006.01) B01L 99/00 (2010.01) B01L 3/00 (2006.01) B25J 5/00 (2006.01) B25J 11/00 (2006.01) C12M 3/00 (2006.01) C12M 3/04 (2006.01)

Patent

CA 2435814

A fermentation apparatus is described to produce known and repeatable amount of untainted fermentation product using multiple fermentation vessels. To facilitate compatible processing with other processing steps, the fermentation apparatus has an array of sample vessels arranged in a container frame (Figure 14). The container frame is configured to hold the sample vessels during fermentation and transport of the vessel array to, or from another processing station. Corresponding to the number of sample vessels in the sample vessel array, a cannula may be placed inside a sample vessel. The cannula array is attached to a gas distributor that delivers oxygen and/or one or more other gases from a gas source through the cannula into the sample vessel. Because of its design, this fermentation apparatus is applicable for simultaneous optimization of a number of process parameters (e.g., cell growth rates).

Cette invention concerne une installation de fermentation capable de produire de façon répétée des quantités connues d'un produit de fermentation sans colorant au moyen de récipients de fermentation multiples. Pour une meilleure compatibilité avec d'autres opérations du traitement, l'installation de fermentation comprend une batterie de récipients pour échantillons disposés dans un cadre de rangement (Figure 14). Ce cadre assure le maintien des récipients pour échantillons pendant la fermentation et leur transport à destination ou en provenance d'un autre poste de traitement. Chaque récipient pour échantillon de la batterie peut recevoir une canule. La batterie de canules est reliée à un distributeur de gaz qui fournit aux récipients, via la canule, de l'oxygène et/ou un ou plusieurs autres gaz. De par sa conception, cette installation de fermentation permet d'optimiser simultanément divers paramètres de fonctionnement (tels que les taux de croissance cellulaire).

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