Sensor constructs and detection methods

Details Sensor constructs and detection methods Sensor constructs and detection methods

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2559719

Sensor constructs 10 for detecting the presence of an analyte 60 in a sample comprise a molecular scaffold 20, a pair of labels 40, 50 which interact via Förster resonance energy transfer, and at least one molecular recognition domain 30. When the analyte 60 is bound by the molecular recognition domain 30, the Förster resonance energy interaction between the pair of labels 40, 50 is disrupted, resulting in a changed optical signal.

L'invention concerne des constructions de capteurs (10) destinés à détecter la présence d'un analyte (60) dans un échantillon. Ces constructions comprennent un échafaudage moléculaire (20), une paire d'étiquettes (40, 50) qui interagissent par l'intermédiaire d'un transfert d'énergie de résonance Förster et au moins un domaine de reconnaissance moléculaire (30). Lorsque l'analyte (60) est lié au domaine de reconnaissance moléculaire (30), l'interaction d'énergie de résonance Förster entre la paire d'étiquettes (40, 50) est déréglée, ce qui a pour conséquence un signal optique modifié.

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