Method for testing a cell suspension

Details Method for testing a cell suspension Method for testing a cell suspension

G - Physics

01

N

G01N 33/487 (2006.01) G01N 13/04 (2006.01) G01N 15/02 (2006.01) G01N 33/49 (2006.01)

Patent

CA 2240475

A method for testing a cell suspension comprises the steps of: (1) diluting a portion of the cell suspension such that the liquid of the diluted suspension has the same osmolality as the extra cellular liquid of the cell suspension (plasma), and (2) subjecting the cells in the diluted suspension formed in step (1) to a cell size measurement while suspended in said diluted suspension. The method involves the testing of cells at their in vivo osmolality, thereby minimizing the possibility of swelling over time and mimicking the cells in vivo condition in respect of its osmotic environment.

Un procédé pour tester une suspension de cellules consiste à: (1) diluer une portion de la suspension de cellules pour que la suspension liquide diluée ait la même osmolalité que le liquide extra-cellulaire de la suspension de cellules (plasma) et (2) soumettre les cellules dans la suspension diluée formée à l'étape (1) à une mesure de la taille, pendant qu'elles sont en suspension diluée. Le procédé consiste à tester les cellules à l'osmolalité qui existe in vivo, ce qui minimise la possibilité de gonflement avec le temps et permet de simuler les conditions in vivo, du point de vue de l'environnement osmotique.

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