Primer extension methods for detecting nucleic acids

Details Primer extension methods for detecting nucleic acids Primer extension methods for detecting nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2326697

Methods are provided for selective nucleic acid sequence detection in single base primer extension reactions of high sensitivity. These methods are useful for detecting small amounts of mutant nucleic acid in a heterogeneous biological sample. These methods are particularly useful for identifying individuals with gene mutations indicative of early colorectal cancer.

L'invention concerne des méthodes de détection d'une séquence d'acide nucléique sélective dans des réactions hautement sensibles d'extension d'amorces en base unique. Ces méthodes s'utilisent pour la détection de petites quantités d'acide nucléique mutant dans un échantillon biologique hétérogène. Ces méthodes sont particulièrement utiles pour l'identification d'individus présentant des mutations de gènes indiquant un cancer colorectal précoce.

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