Selective extraction of dna from groups of cells

Details Selective extraction of dna from groups of cells Selective extraction of dna from groups of cells

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2477017

The invention is in the area of selective extraction of DNA from groups of cells. Selective lysis of a particular cell type within a cellular mixture is performed and then the mixture is separated with a filter that allows the DNA from the lysed cells to flow through the filter, while not allowing the unlysed cells to pass through, thereby selectively extracting the DNA from a particular cell type. In one specific embodiment, spermatozoa DNA can be isolated from biological samples which also contain epithelial cells. Methods and kits are also provided which allow for the sequential extraction of DNA from mixtures of cells. The DNA in the sample can be from human, animal or vegetal origin, or any combination of human, animal or vegetal DNA.

L'invention a trait au domaine de l'extraction sélective d'ADN de groupes de cellules. Une lyse sélective d'un type de cellules particulier est mise en oeuvre dans un mélange cellulaire, puis le mélange est séparé à l'aide d'un filtre qui laisse l'ADN provenant des cellules lysées s'écouler à travers le filtre et retient les cellules non lysées, ce qui permet d'extraire sélectivement l'ADN provenant d'un type de cellules particulier. Dans une forme de réalisation spécifique, l'ADN de spermatozoïdes peut être isolé d'échantillons biologiques contenant également des cellules épithéliales. L'invention concerne aussi des procédés et des trousses permettant d'extraire de façon séquentielle l'ADN de mélanges de cellules. L'ADN de l'échantillon peut être d'origine humaine, animale ou végétale, ou peut être une combinaison d'ADN d'origine humaine, animale ou végétale.

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