Method of identifying polypeptides

Details Method of identifying polypeptides Method of identifying polypeptides

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) C12N 11/02 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2244947

Polypeptides which have been separated by gel electrophoresis can be identified by electroblotting them through a "sandwich" comprising in order: a) the separation gel; b) at least one hydrophilic membrane, e.g. of carboxyl-modified PVDF, on which is immobilised at least one reagent capable of cleaving a polypeptide, e.g. trypsin; c) a hydrophobic layer, typically a membrane, e.g. of PVDF Preferably a biased alternating current or discontinuous direct current is used for electroblotting. The resulting fragments, usually peptides, are identified, preferably by MALDI-TOF MS.

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