G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/545 (2006.01) C07H 21/00 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2491691
Provided are protein microarrays, their manufacture, use, and application. Protein microarrays in accordance with the present invention are useful in a variety preoteomic analyses. Various protein arrays in accordance with the present invention may immobilize large arrays of proteins that may be useful for studying protein-protein interactions to improve understanding of disease processes, facilitating drug discovery, or for identifying potential antigens for vaccine development. The protein array elements of the invention are native or modified proteins (e.g., antibodies or fusion proteins). The protein array elements may be attached directly to a organic functionalized mirrored substrate by a binding reaction between functional groups on the substrate (e.g., amine) and protein (e.g., activated carboxylic acid). Techniques for chemical blocking of the arrays are also provided. The invention contemplates spotting of array elements onto solid planar substrates, labeling of complex protein mixtures, and the analysis of protein binding to the array. The invention also enables the enrichment or purification, and subsequent sequencing or structural analysis of proteins that are identified as differential by the array screen. Kits including protein-binding microarrays for proteomic analysis in accordance with the present invention are also provided.
L'invention concerne des micror~seaux prot~iques ainsi que leur fabrication, utilisation et application. Les mircror~seaux prot~iques de l'invention conviennent pour diverses analyses prot~omiques. Divers micror~seaux prot~iques selon l'invention peuvent immobiliser de vastes ensembles de prot~ines pouvant Útre utiles pour l'~tude d'interactions inter-prot~ines et am~liorer par l~ la compr~hension e processus pathologiques, faciliter la d~couverte de m~dicaments ou identifier des antignes potentiels en vue de l'~laboration de vaccins. Les ~l~ments de r~seau prot~ique de l'invention sont des prot~ines natives ou modifi~es (telles que des anticorps ou des prot~ines de fusion). Les ~l~ments de r~seau prot~iques peut Útre reli~s directement ~ un substrat organique fonctionnalis~ en miroir par une r~action de liaison entre des groupes fonctionnels sur le substrat (ex. : amine) et la prot~ine (ex. :acide carboxylique activ~). L'invention porte ~galement sur des techniques de blocage chimique des r~seaux. Elle concerne le rep~rage d'~l~ments de r~seau sur des substrats solides plans, le marquage de m~langes prot~iques complexes et l'analyse de liaisons prot~iques avec le r~seau. Cette invention permet ~galement de proc~der ~ un enrichissement ou ~ une purification ainsi qu'au s~quen×age ou ~ l'analyse structurelle ult~rieurs de prot~ines identifi~es comme diff~rentielles par le criblage du r~seau. Sont ~galement d~crites des trousses renfermant les micror~seaux prot~omiques selon la pr~sente invention.
Charych Deborah
Zuckermann Ronald N.
Borden Ladner Gervais Llp
Chiron Corporation
Novartis Vaccines And Diagnostics Inc.
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