C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/073 (2010.01) C12N 5/0735 (2010.01) C12N 5/0775 (2010.01) A61K 35/44 (2006.01)
Patent
CA 2577447
The present invention relates to a method for isolating stem/progenitor cells from the amniotic membrane of umbilical cord, wherein the method comprises separating the amniotic membrane from the other components of the umbilical cord in vitro, culturing the amniotic membrane tissue under conditions allowing cell proliferation, and isolating the stem/progenitor cells from the tissue cultures. The isolated stem cell cells can have embryonic stem cell- like properties and can be used for various therapeutic purposes. In one embodiment, the invention relates to the isolation and cultivation of stem cells such as epithelial and/or mesenchymal stem/progenitor cells under conditions allowing the cells to undergo mitotic expansion. Furthermore, the invention is directed to a method for the differentiation of the isolated stem/progenitor cells into epithelial and/or mesenchymal cells.
L'invention concerne un procédé d'isolement de cellules souches/progénitrices issues de la membrane amniotique du cordon ombilical. Le procédé comporte les étapes consistant à: séparer in vitro la membrane amniotique des autres composants du cordon ombilical, mettre en culture le tissu de membrane amniotique dans des conditions permettant une prolifération cellulaire, et isoler les cellules souches/progénitrices des cultures de tissu. Les cellules souches isolées possèdent des propriétés analogues à celles des cellules souches et peuvent être utilisées à diverses fins thérapeutiques. Un mode de réalisation de l'invention concerne l'isolement et la mise en culture de cellules souches telles que des cellules épithéliales et/ou des cellules souches/progénitrices mésenchymateuses, dans des conditions permettant la mitose desdites cellules. De plus, l'invention concerne un procédé de différenciation des cellules souches/progénitrices isolées en cellules épithéliales et/ou mésenchymateuses.
Lim Ivor Jiun
Phan Toan-Thang
Blake Cassels & Graydon Llp
Cellresearch Corporation Pte Ltd
LandOfFree
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