Dna sequencing by mass spectrometry via exonuclease degradation

Details Dna sequencing by mass spectrometry via exonuclease degradation Dna sequencing by mass spectrometry via exonuclease degradation

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 19/06 (2006.01) C07H 19/10 (2006.01) C07H 19/16 (2006.01) C07H 19/20 (2006.01) C07H 21/00 (2006.01) C12M 1/40 (2006.01) G01N 35/10 (2006.01)

Patent

CA 2158642

Methods for determining the sequence of nucleic acids by cleaving the nucleic acid unilaterally from a first end with an exonuclease activity to sequentially release individual nucleotides, identifying each of the sequentially release nucleotides by mass spectometry, and determining the sequence of the nucleic acid from the identified nucleotides are disclosed. The method is amenable to multiplexing for simultaneously determining more than one nucleic acid sequence.

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