Site specific incorporation of keto amino acids into proteins

Details Site specific incorporation of keto amino acids into proteins Site specific incorporation of keto amino acids into proteins

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) C12N 1/20 (2006.01) C12N 9/00 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2502029

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.

L'invention concerne des compositions et des procédés de production d'une machinerie biosynthétique pour protéines, comprenant des ARNt orthogonaux, des aminoacyl-ARNt synthétases orthogonales et des paires orthogonales d'ARNt/synthétases, qui permettent d'incorporer des aminoacides-cétones dans des protéines. Des procédés d'identification de ces paires orthogonales sont également prévus conjointement avec des méthodes de production de protéines comprenant des aminoacides-cétones, au moyen de ces paires orthogonales.

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