Template-mediated, ligation-oriented method of nonrandomly...

Details Template-mediated, ligation-oriented method of nonrandomly... Template-mediated, ligation-oriented method of nonrandomly...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2445258

Method of gene shuffling oriented ligation, whereby at least two fragments are adjacently hybridized on an assembly template. Invention is particularly aimed at generating novel polynucleotides that differ in some advantageous respect compared to a reference sequence. Invention further includes sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.

L'invention concerne un procédé de réarrangement génétique faisant intervenir la ligature orientée, consistant à hybrider au moins deux fragments de manière adjacente sur une matrice d'assemblage. La présente invention vise notamment à créer de nouveaux polynucléotides présentant des caractéristiques avantageuses par rapport à une séquence de référence. La présente invention concerne également des séquences créées selon ledit procédé, des hôtes et des vecteurs contenant lesdites séquences, ainsi que des protéines traduites à partir desdites séquences.

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