Method of detoxication of yperite by using haloalkane...

A - Human Necessities – 62 – D

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A62D 3/00 (2007.01) B09C 1/10 (2006.01) C02F 3/34 (2006.01) C02F 11/00 (2006.01) C02F 11/02 (2006.01)

Patent

CA 2610024

The method of detoxification of yperite - bis(2-chloroethyl) sulfide - by the use of a haloalkane dehalogenases or their compositions, the method of preparation of dehalogenationating enzymes and of decontamination compositions which contains at least one wild type and/or modified haloalkane dehalogenase (EC 3.8.1.5) as an chemically active component. The preferred dehalogenases are LJnB from Sphingomonas paucimobilis UT26, DhaA from Rhodococcus rodochrous NCIMB 13064, DmbA from Mycobacterium bovis 5033/66. Decontamination is utilizable for detoxification of yperite from the surfaces of instrumentality, constructional objects, human or animal skin and elements of environment by the treatment of yperite with the detoxification composition according the invention at +10~C to +70~C, preferably at about +40~C and pH from 4 to 12.

L'invention concerne un procédé destiné à la détoxication de l'ypérite - sulfure de bis(2-chloroéthyle) -, dans lequel des haloalcanes déshalogénases ou des compositions à base d'haloalcanes déshalogénases sont utilisées. L'invention concerne également un procédé destiné à la préparation d'enzymes de déshalogénation et de compositions de décontamination contenant au moins une haloalcane déshalogénase de type sauvage et/ou modifiée (EC 3.8.1.5) en tant que composant chimiquement actif. Les déshalogénases préférées sont la LJnB de Sphingomonas paucimobilis UT26, DhaA deRhodococcus rodochrous NCIMB 13064, DmbA de Mycobacterium bovis 5033/66. La décontamination peut être utilisée pour détoxiquer l'ypérite située sur la surface d'instruments, d'objets de construction, de la peau animale ou humaine et d'éléments de l'environnement. La décontamination se fait à l'aide de la composition de détoxication selon l'invention, à une température comprise entre +10 °C et +70 °C, de préférence +40 °C environ, et à un pH compris entre 4 et 12.

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